Guidance Document
Carcinogenic Substances

Alkylating agents
Hydrazines

a-Halo ethers
Hydrazine (and hydrazine salts)

Bis(chloromethyl) ether
1,2-Diethylhydrazine

Methyl chloromethyl ether
1,1-Dimethylhydrazine

Sulfonates
1,2-Dimethylhydrazine

1,4-Butanediol dimethanesulfonate (myleran)


Diethyl sulfate
N-Nitroso compounds

Dimethyl sulfate
N-Nitrosodimethylamine

Ethyl methanesulfonate
N-Nitroso-N-alkylurea

Methyl methanesulfonate


Methyl trifluoromethanesulfonate
Aromatic amines

1,3-Propanesultone
4-Aminobiphenyl

Epoxides
Benzidine (4, 4'-diaminobiphenyl)

Ethylene oxide
a-Naphthylamine

Diepoxybutane
b-Naphthylamine

Epichlorohydrin
Aniline

Propylene oxide
o-Anisidine(2-methoxyaniline)

Styrene oxide
2,4-Diaminotoluene

Aziridines
o-Toluidine

Ethylenimine


2-methylaziridine
Aromatic hydrocarbons

Diazo, azo, and azoxy compounds
Benzene

4-Dimethylaminoazobenzene
Benz[a]anthracene

Electrophilic alkenes and alkynes
Benzo[a]pyrene

Acrylonitrile


Acrolein
Natural products (including antitumor drugs)
Ethyl acrylate
Adriamycin


Aflatoxins


Acylating agents
Bleomycin

b-Propiolactone
Cisplatin

b-Butyrolactone
Progesterone

Dimethylcarbamyl chloride
Reserpine


Safrole

Organohalogen compounds


1,2-Dibromo-3-chloropropane
Miscellaneous organic compounds

Mustard gas (bis(2-chloroethyl)sulfide)
Formaldehyde (gas)

Vinyl chloride
Acetaldehyde

Carbon tetrachloride
1,4-Dioxane

Chloroform
Ethyl carbamate (uret

3-Chloro-2-methylpropene
Hexamethylphosphor

1,2-Dibromoethane
2-Nitropropane

1,4-Dichlorobenzene
Styrene

1,2-Dichloroethane
Thiourea

2,2-Dichloroethane
Thioacetamide

1,3-Dichloropropene


Hexachlorobenzene
Miscellaneous inorganic compounds

Methyl iodide
Arsenic and certain arsenic compounds

Tetrachloroethylene
Chromium and certain chromium compounds

Trichloroethylene
Thorium dioxide

2,4,6-Trichlorophenol
Beryllium and certain beryllium compounds


Cadmium and certain cadmium compounds


Lead and certain lead compounds


Nickel and certain nickel compounds


Selenium sulfide


From Prudent Practices in the Laboratory, National Academy Press, 1995.