CHEMICAL HYGIENE PROGRAM

Foreword
2
1.0 Chemical Hygiene Responsibilities and Program Coverage
2
1.2. Scope and Application of this Plan
4
1.3. Coordination with Other Standards and Guidelines
4
2.0 Employee Information and Training
4
2.1 Information
4
2.2 Training
5
3.0 Criteria for Implementation of Control Measures
6
3.1 General Criteria
6
3.2 Criteria for Implementation of Specific Control Measures
6
4.0 Management of Engineering Controls
9
5.0 Standard Operating Procedures for Laboratory Chemicals
10
5.1 General Principles
10
6.0 Particularly Hazardous Procedures
11
6.1 Work with Particularly Hazardous Substances
11
6.2 Pre-approval of Particularly Hazardous Work
12
7.0 Chemical Spills, Releases and Accidents
13
7.1 Emergency Response
13
7.2 In Case of Fire
13
7.3 In Case of Spills
13
7.4 In Case of Personnel Exposures
13
7.5 Emergency Phone Numbers
13
8.0 Medical Consultations and Examinations
14
8.1 Availability
14
8.2 Arranging for Exams
14
8.3 Information
14
8.4 Report
14
9.0 Recordkeeping
15
10.0 Annual Chemical Hygiene Plan Review
15
11.0 References and Other Information Sources
16
A. Literature References
16
Table of Contents of Prudent Practices in the Laboratory
17
B. Internet Resources
18
Appendix A: Laboratory Specific Chemical Hygiene Procedures
19
Instructions for Chemical Hygiene Procedures Form
21
PERMIT FOR USE OF NON-STANDARD LABORATORY OR SPECIAL OPERATING PROCEDURES

23
Appendix B: Work with High Hazard Chemicals
24
Table 1: Carcinogens, Reproductive Toxins or Highly Toxic Chemicals
24



CHEMICAL HYGIENE PLAN (rev. 7/03/2012)

Page 1


Foreword
The protection of the safety and health of its employees, students and environment is a
high priority of the Colorado School of Mines (Mines or the School). On January 31,
1990, the Occupational Safety and Health Administration (OSHA) promulgated a rule
related to occupational exposures to hazardous chemicals in laboratories. This rule is
designed to help protect laboratory workers from the hazards of the chemicals they use.
Included in the standard is a requirement that all employers covered by the standard
develop a Chemical Hygiene Plan (CHP). A CHP is a written program which sets forth
work practices, equipment use and maintenance procedures, and personal protective
equipment requirements that protect employees from the hazards presented by
chemicals used in the lab.
According to OSHA, the CHP must include standard operating procedures, criteria for
the implementation of chemical control measures, measures to ensure proper operation
of engineering controls, provisions for the training of workers, provisions for medical
consultation in the case of exposure, designation of responsible people in the lab, and
identification of procedures for the use of particularly hazardous substances or
procedures. This document satisfies this requirement.
It is up to each lab supervisor to supplement this plan with more detailed information
about the proper use of the particular chemicals used in their lab. These supplements
may be in the form of written procedures, literature libraries, video presentations, and/or
group or individual training. The lab supervisor and Chemical Hygiene Officer, if one is
appointed, are responsible for the interpretation and enforcement of policies described
in this CHP. The Environmental Health & Safety staff is available to provide technical
assistance with this effort. Contact us at (303) 273-3316 or mberton@mines.edu for
more information.
1.0 Chemical Hygiene Responsibilities and Program
Coverage
1.1 Chemical Hygiene Responsibilities
Duties specific to laboratory chemical use are described in this section.
A. Laboratory Supervisor
The laboratory supervisor has the ultimate responsibility for chemical hygiene
throughout the laboratory, and, with the assistance of campus laboratory safety
programs, supports the chemical hygiene efforts of lab workers.


CHEMICAL HYGIENE PLAN (rev. 7/03/2012)

Page 2

Specifically, the lab supervisor shall:
 Develop and implement appropriate chemical hygiene policies and practices
specific to the operations of the lab(s) they are responsible for. The form included
in Appendix A is designed to provide a mechanism to assist with this work.
 Perform regular, formal chemical hygiene inspections, including inspections of
emergency equipment. The frequency of these will be set by the laboratory CHP,
based on the professional judgment of the lab supervisor. Weekly housekeeping
inspections and monthly equipment inspections are suggested.
 Develop Standard Operating Procedures (SOPs) specific to their lab's
operations.
 Determine the proper level and type of personal protective equipment for lab
operations.
 Ensure that appropriate training has been provided to employees.
 Maintain a current knowledge concerning the legal requirements of regulated
substances in the laboratory.
 Review and improve the Chemical Hygiene Plan on an annual basis.
B. Chemical Hygiene Officer
The lab supervisor may name a Chemical Hygiene Officer (CHO) with appropriate
training and experience to assist with the activities described above. If no person is
named CHO, the lab supervisor will retain responsibility for all chemical hygiene
activities.
C. Laboratory Workers
The laboratory workers are individually responsible for planning and conducting each
laboratory operation in accordance with the Chemical Hygiene Plan and developing
good personal chemical hygiene habits.
D. Environmental Health & Safety Department
The Environmental Health and Safety Department (EHS) offers a range of chemical
health and safety issues services. These services are designed to provide general
assistance in meeting regulatory compliance and safety concerns. They need to be
supplemented by laboratory-specific safety programs in order to achieve full regulatory
compliance.
E. Chemical Hygiene Committee
The Mines Safety Committee will act as the University Chemical Hygiene Committee. In
this role, it will provide technical and policy oversight of laboratory activities which
involve the use of hazardous chemicals.


CHEMICAL HYGIENE PLAN (rev. 7/03/2012)

Page 3

1.2. Scope and Application of this Plan
This standard applies where "laboratory use" of hazardous chemicals occurs.
Laboratory use of hazardous chemicals means handling or use of such chemicals in
which all of the following conditions are met:
 the handling or use of chemicals occurs on a "laboratory scale"; that is, the work
involves containers which can easily and safely be manipulated by one person;
 multiple chemical procedures or chemical substances are used; and
 protective laboratory practices and equipment are available and in common use
to minimize the potential for employee exposures to hazardous chemicals.
At a minimum, this definition covers employees (including student employees,
technicians, research faculty, and principal investigators) who use chemicals in teaching
and research laboratories at the School. Certain non-traditional laboratory settings may
be included under this standard at the option of individual departments within the
University. Also, it is the policy of the School that laboratory students, while not legally
covered under this standard, will be given training commensurate with the level of
hazard associated with their laboratory work.
Where the use of hazardous chemicals provides no potential for employee exposure,
such as in procedures using chemically impregnated test media and commercially
prepared test kits, a Chemical Hygiene Plan is not required.

1.3. Coordination with Other Standards and Guidelines
Although this standard deals only with use of hazardous chemicals, employees may
also encounter potential physical, biological or radioactive hazards in the laboratory. In
addition, other campus policies and procedures affect the use of hazardous chemicals.
For example, the Mines Hazardous Waste Management Plan describes the proper
procedures for the disposal of laboratory chemicals. In the event that there is a conflict
between provisions of various standards, the Environmental Health & Safety
Department (EHS) should be contacted to assist in resolving the discrepancy.

2.0 Employee Information and Training
2.1 Information
It is essential that laboratory employees have access to information on the hazards of
chemicals and procedures for working safely. Supervisors must ensure that laboratory
employees are informed about and have access to the following information sources:

CHEMICAL HYGIENE PLAN (rev. 7/03/2012)

Page 4

 The contents of the OSHA lab standard, Occupational Exposure to Hazardous
Chemicals in Laboratories, and its appendices (29 CFR 1910.1450).
 The Mines Chemical Hygiene Plan (this document) and local lab standard
operating procedures.
 The Permissible Exposure Limits (PEL) for OSHA regulated substances.
 Material Safety Data Sheets (MSDS) for laboratory chemicals. These are
available from collections at the Chemical Storage and Distribution Facility, on
the Internet (http://hazard.com/msds), and are also located in many individual
laboratories. Departments will make such information available to the employees
using the chemicals.


2.2 Training
Each laboratory supervisor is responsible for ensuring that laboratory employees are
provided with training about the hazards of chemicals present in their laboratory work
area, and methods to control exposure to such chemicals. Each employee shall receive
training at the time of initial assignment to the laboratory, prior to assignments involving
new exposure situations, and at a regular frequency.
A. Availability
Training is available in the form of:
 Literature describing proper lab practices (see Sections 5 and11).
 Video libraries at the EHS and in the departments.
 Group and individual training, conducted by lab personnel or EHS staff.
B. Content
Employee training programs will include, at a minimum, the following subjects:
 Methods of detecting the presence of hazardous chemicals (observation, signage
and labeling, odor, real-time monitoring, air sampling, etc.).
 Symptoms associated with exposures to hazardous chemicals.
 Good laboratory practice, including general techniques designed to reduce
personal exposure and to control physical hazards, as well as specific protective
mechanisms and warning systems used in individual laboratories.
 Emergency response actions appropriate to individual laboratories.
 Applicable details of the departmental Chemical Hygiene Plan, including general
and laboratory-specific Standard Operating Procedures.
 An introduction to the Mines Hazardous Waste Management procedures.



CHEMICAL HYGIENE PLAN (rev. 7/03/2012)

Page 5

3.0 Criteria for Implementation of Control Measures
3.1 General Criteria
This Chemical Hygiene Plan is intended to limit laboratory workers' exposure to OSHA-
regulated substances. Laboratory workers must not be exposed to substances in
excess of the permissible exposure limits (PEL) specified in OSHA rule 29 CFR 1910,
Subpart Z, Toxic and Hazardous Substances or Threshold Limits Values set by the
American Conference of Governmental Industrial Hygienists. PELs refer to airborne
concentrations of substances and are averaged over an eight-hour day. A few
substances also have "action levels". Action levels are air concentrations below the
PEL which nevertheless require that certain actions such as medical surveillance and
workplace monitoring take place.
An employee's workplace exposure to any regulated substance must be monitored if
there is reason to believe that the exposure will exceed an action level or a PEL. If
exposures to any regulated substance routinely exceed an action level or permissible
exposure level, control measures must be implemented.
A. Professional Judgment
The lab supervisor can use professional judgment to assess the nature of chemical
exposure resulting from a lab procedure and prescribe engineering controls and
personal protective equipment to be used during the procedure. This judgment will be
documented through use of Standard Operating Procedures and Laboratory Chemical
Safety Summaries written for the chemicals in use.
B. Air Sampling
Air sampling for evaluating employee exposure to chemical substances shall be
conducted on an as needed basis (to be determined by the lab supervisor). Conduct air
sampling if there is reason to believe that exposure levels for regulated substances that
require sampling routinely exceed the action level, or in the absence of an action level,
the PEL.
Air sampling will be conducted according to established industrial hygiene practices. It
may be conducted by lab workers, EHS staff or outside consultants. The results of air
sampling studies performed in the laboratory should be sent to the Environmental
Health and Safety Department for records maintenance.
3.2 Criteria for Implementation of Specific Control Measures
Engineering controls, personal protective equipment, hygiene practices, and
administrative controls each play a role in a comprehensive laboratory safety program.
Implementation of specific measures must be carried out on a case-by-case basis,
using the following criteria for guidance in making decisions.
A. When to Use Fume Hoods

CHEMICAL HYGIENE PLAN (rev. 7/03/2012)

Page 6

The laboratory fume hood is the major protective device available to laboratory workers.
It is designed to capture chemicals that escape from their containers or apparatus and
to remove them from the laboratory environment before they can be inhaled.
Characteristics to be considered in requiring fume hood use are physical state, volatility,
toxicity, flammability, eye and skin irritation, odor, and the potential for producing
aerosols. A fume hood should be used if a proposed chemical procedure exhibits any
one of these characteristics to a degree that
1. airborne concentrations might approach the action level (or permissible exposure
limit),
2. flammable vapors might approach one tenth of the lower explosion limit,
3. materials of unknown toxicity are used or generated, or
4. the odor produced is annoying to laboratory occupants or adjacent units.
Procedures that can generally be carried out safely outside the fume hood (depending
on the capacity of the general ventilation system to remove any airborne contaminants)
include those involving:
1. water-based solutions of salts, dilute acids, bases, or other reagents,
2. very low volatility liquids or solids,
3. closed systems that do not allow significant escape to the laboratory environment,
and
4. extremely small quantities of otherwise problematic chemicals.
The procedure itself must be evaluated for its potential to increase volatility or produce
aerosols.
B. When to Use Safety Shields or Other Containment Devices
Safety shields, such as the sliding sash of a fume hood, are appropriate when working
with highly concentrated acids, bases, oxidizers or reducing agents, all of which have
the potential for causing sudden spattering or even explosive release of material.
Reactions carried out at non-ambient pressures (vacuum or high pressure) also require
safety shields, as do reactions that are carried out for the first time or are significantly
scaled up from normal conditions.
Other containment devices, such as glove boxes or vented gas cabinets, may be
required when it is necessary to provide an inert atmosphere for the chemical procedure
taking place, when capture of any chemical emission is desirable, or when the standard
laboratory fume hood does not provide adequate assurance that overexposure to a
hazardous chemical will not occur. The presence of biological or radioactive materials
may also mandate certain special containment devices.
Local exhaust ventilation may be required for equipment that exhausts toxic or irritating
materials to the laboratory environment.
Ventilated chemical storage cabinets or rooms should be used when the chemicals in
storage may generate toxic, flammable or irritating levels of airborne contamination.

CHEMICAL HYGIENE PLAN (rev. 7/03/2012)

Page 7

C. When to Use Personal Protective Equipment
Laboratory supervisors or CHO's shall designate areas, activities, and tasks which
require specific types of personal protective equipment. Protective equipment shall not
be worn in public areas, in order to prevent the spread of chemical or biological
contamination from laboratory areas.
Eye Protection
Eye protection is required for all personnel and any visitors whose eyes may be
exposed to chemical or physical hazards. Side shields on safety spectacles provide
some protection against splashed chemicals or flying particles, but goggles or face
shields are necessary when there is a greater than average danger of eye contact. A
higher than average risk exists when working with highly reactive chemicals,
concentrated corrosives, or with vacuum or pressurized glassware systems.
Protective Clothing
Lab coats or other similar clothing protectors are strongly encouraged for all laboratory
personnel. Lab coats are required when working with select carcinogens, reproductive
toxins, substances which have a high degree of acute toxicity, strong acids and bases,
and any substance on the OSHA PEL list carrying a "skin" notation.
Bare feet are not permitted in any laboratory. Sandals and open-toed shoes are
strongly discouraged in all laboratories and are not permitted in any situation where lab
coats or gloves are required.
Gloves
Gloves made of appropriate material are required to protect the hands and arms from
thermal burns, cuts, or chemical exposure that may result in absorption through the skin
or reaction on the surface of the skin. Gloves are also required when working with
particularly hazardous substances where possible transfer from hand to mouth must be
avoided.
Gloves should be carefully selected using guides from the manufacturers. General
selection guides are available; however, glove resistance to chemicals will vary with the
manufacturer, model and thickness. Therefore, review a glove-resistance chart from
the manufacturer you intend to buy from, before purchasing gloves.
Respiratory Protection
Respiratory protection is generally not necessary in the laboratory setting and must not
be used as a substitute for adequate engineering controls. Availability of respiratory
protection for emergency situations may be required when working with chemicals that
are highly toxic and highly volatile or gaseous. If an experimental protocol requires
exposure above the action level that cannot be reduced, respiratory protection will be
required. All use of respiratory protective equipment is covered under the Mines
Respiratory Protection Program.

CHEMICAL HYGIENE PLAN (rev. 7/03/2012)

Page 8

4.0 Management of Engineering Controls
The engineering controls installed in the laboratory are intended to minimize employee
exposure to chemical and physical hazards in the workplace. These controls must be
maintained in proper working order for this goal to be realized.
No modification of engineering controls will occur unless testing of the modification
indicates that worker protection will continue to be adequate. Improper function of
engineering controls must be reported to the lab supervisor immediately. The system
shall be taken out of service until proper repairs have been executed.
4.1 Local Exhaust Ventilation
The following procedures shall apply to the use of local exhaust ventilation:
 Openings of local exhaust will be as close as possible to the source of the
contaminants.
 Local exhaust fans shall be turned on when exhaust hoods are being used.
 After using local exhaust, operate the fan for an additional period of time
sufficient to clear residual contaminants from the ductwork.
 The ventilation system shall be inspected annually by the Plant Facilities
Department.
 Prior to a change in chemicals or procedures, the adequacy of the available
ventilation systems shall be determined by the lab supervisor.
4.2 Laboratory Hoods
Prior to the introduction of new chemicals, the adequacy of hood systems available shall
be determined by the lab supervisor.
Ductless fume hoods recirculate exhaust air through filters back into the room.
Therefore, they can not be used for volatile toxic materials and should be posted as
"Not for use with toxic materials." Consult EHS staff before using these hoods to control
air contaminants.
4.3 Chemical Storage Cabinets
Storage cabinets for flammable and hazardous chemicals will be ventilated as needed.
They will be provided with a spill containment system appropriate to the chemicals
stored in them.
4.4 Biosafety Cabinets, Glove Boxes and Isolation Rooms
The exhaust air from a biosafety cabinets, glove box or isolation room will pass through
scrubbers, HEPA filters, or other treatment before release into the regular exhaust
system. Biosafety cabinets will be certified annually and each time they are moved.
This certification is arranged by the EHS Department.

CHEMICAL HYGIENE PLAN (rev. 7/03/2012)

Page 9

4.5 Cold Rooms and Warm Rooms
Temperature control rooms generally do not have fresh air ventilation. Do not use
volatile chemicals in them! Also note that liquid nitrogen stored in these rooms can
displace oxygen and cause oxygen deficient conditions.
4.6 Emergency Equipment
Eye washes must be flushed weekly by the user. This will ensure that the eye wash is
working, and that the water is clean, should emergency use become necessary. Fire
extinguishers are checked annually by the EHS Department.
5.0 Standard Operating Procedures for Laboratory
Chemicals
Standard Operating Procedures (SOPs) are generally accepted practices for use of
chemicals in particular situations. These SOPs can be overridden in specific instances
when appropriate. It is advisable to document the reasons for such modifications. When
SOPs are not available for a specific lab situation, the lab supervisor and CHP will
develop them, in consultation with the references cited below and the EHS staff.
5.1 General Principles
A. Controlling Chemical Exposure
Each laboratory employee shall minimize personal and coworker exposure to the
chemicals in the laboratory. General precautions which shall be followed to achieve this
goal during the handling and use of all chemicals are as follows:
 A chemical mixture shall be assumed to be as toxic as its most toxic component.
Possibilities for substitution will be investigated.
 Laboratory employees shall be familiar with the symptoms of exposure for the
chemicals with which they work and the precautions necessary to prevent
exposure.
 Eating, drinking, and smoking are prohibited in areas where laboratory chemicals
are present. Hands shall be thoroughly washed after working with chemicals.
Storage, handling and consumption of food or beverages shall not occur in
chemical storage areas, nor refrigerators, nor with glassware or utensils also
used for laboratory operations.
 Each employee shall keep the work area clean and uncluttered. All chemicals
and equipment shall be labeled with appropriate hazard warnings. At the
completion of each work day or operation, the work area shall be cleaned.
 Mouth suction for pipeting or starting a siphon is prohibited.
 Skin contact with all chemicals shall be avoided. Employees shall wash exposed
skin prior to leaving the laboratory.

CHEMICAL HYGIENE PLAN (rev. 7/03/2012)

Page 10

 Additional specific precautions based on the toxicological characteristics of
individual chemicals shall be implemented as deemed necessary by the lab
supervisor.
B. Laboratory Equipment
The following rules shall apply to the use of laboratory equipment:
 All laboratory equipment shall be used only for its intended purpose.
 All glassware will be handled and stored to minimize breakage; all broken
glassware will be immediately disposed of in the broken glass container.
 All evacuated glass apparatus shall be shielded to contain chemicals and glass
fragments should implosion occur.
 Waste receptacles shall be identified as such by signs attached to the receptacle.
 All laboratory equipment shall be inspected on a periodic basis and replaced or
repaired as necessary.
C. Planning for Emergencies
Before work with laboratory chemicals begins, plans for various emergencies will be
developed. The circumstances to be covered include fire, chemical spill, and personnel
exposure. In addition, the following work practices will be observed:
 Spill containment will be established around areas in which more than one liter of
liquid is used.
 Workers manipulating chemicals will always be in easy communication of other
people while handling chemicals
 Emergency equipment will be checked on a daily basis for unusual conditions.
General information for emergency response at Mines is given in Section 7.

6.0 Particularly Hazardous Procedures
The OSHA Lab Standard requires that special consideration be given to use of
chemicals or procedures with particular hazards. The definition of "particularly
hazardous chemicals" is given in the OSHA lab standard. Examples of such chemicals
are given in Chapter 3 of Prudent Practices. A brief list of examples is in Appendix B.
This consideration requires either the development of special operating procedures or
prior approval of the laboratory supervisor as indicated by a written permit describing
the conditions for the work to be done.
6.1 Work with Particularly Hazardous Substances
When laboratory procedures include the use of highly hazardous chemicals, special
precautions shall be implemented as deemed necessary by the lab supervisor. These

CHEMICAL HYGIENE PLAN (rev. 7/03/2012)

Page 11

precautions will be developed for work with select carcinogens, reproductive toxins and
substances which have a high degree of acute toxicity. Development of these
precautions will consider including the following provisions in the special procedures:
 Establishment of a designated area for the use of the high hazard chemicals.
 Signage and access control to the work area where the chemical is used.
 Special precautions such as use of containment devices such as glove boxes;
isolation of contaminated equipment; practicing good laboratory hygiene; and
prudent transportation of very toxic chemicals.
 Planning for accidents and spills.
 Special storage and waste disposal practices.
Prudent Practices provides detailed recommendations for work with particularly
hazardous substances.
6.2 Pre-approval of Particularly Hazardous Work
A permit system shall be utilized for all laboratory activities which do not follow standard
or special operating procedures. The permit system shall be implemented and enforced
by the laboratory supervisor. These activities include off-hours work, sole occupancy of
lab and unattended operations. A sample permit is given in Appendix B. The toxicity of
the chemicals used, the hazards of the procedures to be done, and the knowledge and
experience of the laboratory workers must be considered in deciding which work will be
allowed with pre-approval.
Off-Hours Work Procedures: Laboratory personnel are not permitted to work after hours
in the lab, except when permit conditions are met and approved by the laboratory
supervisor.
Working Alone: Work shall not be performed in the laboratory when the only person in
the room is the laboratory person performing the work. Under unusual conditions,
crosschecks, periodic security guard checks, or other measures may be taken as
established by a permit and approved by the laboratory supervisor.
Unattended Operations: When laboratory operations are performed which will be
unattended by laboratory personnel (continuous operations, overnight reactions, etc.),
the following procedures will be employed:
 An appropriate permit will be written, approved by the laboratory supervisor, and
posted.
 A sign will be posted at all entrances to the laboratory.
 Precautions shall be made for the interruption of utility service during the
unattended operation (loss of water pressure, electricity, etc.).
 The person responsible for the operation will return to the laboratory at the
conclusion of the operation to assist in the dismantling of the apparatus.


CHEMICAL HYGIENE PLAN (rev. 7/03/2012)

Page 12

7.0 Chemical Spills, Releases and Accidents
7.1 Emergency Response
Telephone numbers of emergency personnel, supervisors and other workers as
deemed appropriate are posted on the lab entrance by the EHS Department. These
signs will be updated annually for accuracy.
7.2 In Case of Fire
The School’s policy is that the first reaction to a fire is to evacuate the occupants of the
building. Fire extinguishers are available in labs and are inspected annually. They may
be used to fight small fires and to assist in evacuation from fire situations. Fire
extinguisher training is available through the EHS Department.
7.3 In Case of Spills
In the event of a chemical spill, release or other accident, lab workers will respond as
outlined in the University Emergency Response plan. The size of the spill and its
hazards will guide the appropriate response. If there is any doubt about the lab worker's
ability to safely clean up the spill, call the EHS at (303) 273-3316 (24 Hours). Note that
proper emergency response depends upon knowledge of the hazards present in the
lab. For this reason, a campus wide inventory of the hazardous chemicals in Mines labs
is conducted annually.
7.4 In Case of Personnel Exposures
All employees shall be instructed in the location and proper usage of emergency
showers and eyewashes. The eyewash and emergency shower shall be inspected and
flushed weekly. In case of medical emergency, call 911 or CSM Public Safety. Note
that medical consultation after such exposures is provided as detailed in Section 8.0.
7.5 Emergency Phone Numbers
 CSM Police (24 hours) 911
 Environmental Health and Safety Department (303) 273-3316
(24 hours)
 Poison Control Center (800) 222-1222



CHEMICAL HYGIENE PLAN (rev. 7/03/2012)

Page 13

8.0 Medical Consultations and Examinations
8.1 Availability
All employees who work with hazardous chemicals will have an opportunity to receive
medical attention, including any follow-up examinations which the examining physician
determines to be necessary, under the following circumstances:

 Whenever an employee develops symptoms associated with a hazardous
chemical to which the employee may have been exposed in the laboratory.
 Where exposure monitoring reveals an exposure level routinely above the action
level or PEL for an OSHA regulated substance for which there are exposure
monitoring and medical surveillance requirements.
 Whenever an event takes place in the work area such as a spill, leak, explosion
or other occurrence resulting in the likelihood of a hazardous exposure.
EHS will be contacted whenever the need for medical consultation or examination
occurs, or when there is uncertainty as to whether any of the above criteria have been
met.
8.2 Arranging for Exams
All medical examinations and consultations will be performed by or under the direct
supervision of a licensed physician and will be provided through EHS, without loss of
pay and at a reasonable time and place. In the event of a life-threatening illness or
injury, dial 911 and request an ambulance.
8.3 Information
The School will provide the examining physician with the following information:
 The identity of the hazardous chemical(s) to which the employee may have been
exposed.
 A description of the conditions under which the exposure occurred including
quantitative exposure data, if available.
 A description of the symptoms of exposure that the employee is experiencing, if
any.
The above information will be collected and transmitted by the lab supervisor and will be
submitted to the EHS as well as to the examining physician.
8.4 Report
The examining physician will provide to the lab supervisor and EHS a written report
including the following:
 Any recommendation for further medical follow-up.

CHEMICAL HYGIENE PLAN (rev. 7/03/2012)

Page 14

 The results of the medical examination and any associated tests.
 Any medical condition which may be revealed in the course of the examination
which may place the employee at increased risk as a result of exposure to a
hazardous chemical found in the workplace.
 A statement that the employee has been informed by the physician of the results
of the consultation or medical examination and any medical condition that may
require further examination or treatment.
The written opinion will not reveal specific findings of diagnoses unrelated to
occupational exposure.
9.0 Recordkeeping
9.1 Accident Reports
Accident investigations will be conducted by EHS with assistance from the lab
supervisor as deemed necessary. Accident reports will be written and retained for 5
years.
9.2 Exposure Evaluations
Any records of exposure evaluation carried out by individual departments will be kept
within the department and also sent to the EHS Department. Raw data will be kept for
one year and summary data for the term of employment plus 30 years.
9.3 Medical Consultation and Examinations
Results of medical consultations and examinations will be kept by EHS for a length of
time specified by the appropriate medical records standard. This time will be at least
the term of employment plus 30 years as required by OSHA.
9.4 Training
Individual employee training records should be kept in the individual's department or
college file for five years.
9.5 Equipment Inspection
Records of inspections of equipment will be maintained for 5 years. Data on annual
fume hood monitoring will be kept in the EHS Department. Fume hood monitoring data
are considered maintenance records and as such the raw data will be kept for one year
and summary data for 5 years.
10.0 Annual Chemical Hygiene Plan Review
The laboratory supervisor and Chemical Hygiene Officer will review the laboratory's
Chemical Hygiene Plan every March. Results will be provided EHS and the department

CHEMICAL HYGIENE PLAN (rev. 7/03/2012)

Page 15

laboratory manager. Laboratory supervisors are responsible for assigning responsibility
for taking corrective action for any deficiency noted.
11.0 References and Other Information Sources
A. Literature References
National Research Council, Prudent Practices for Handling and Disposing
Hazardous Chemicals in Laboratories, National Academy Press, Washington, D.C.,
1995.
Code of Federal Regulations, 29 CFR part 1910 subpart Z section 1910.1450,
Occupational Exposure to Hazardous Chemicals in Laboratories, 1990.
American Chemical Society, Safety in Academy Chemistry Laboratories, 5th ed.,
Washington, D.C., 1991.
Department of Risk Management, UVM Fumehood Operation & Safety Guidelines,
University of Vermont, Burlington, VT, 1991.



CHEMICAL HYGIENE PLAN (rev. 7/03/2012)

Page 16

Table of Contents of Prudent Practices in the Laboratory
Prudent Practices in the Laboratory: Handling and Disposal of Chemicals, National
Academy Press, Washington, D.C. 1995.
Chapter 1: The Culture of Laboratory Safety
The New Culture of Laboratory Safety; Responsibility and Accountability for Laboratory
Safety; Special Safety Considerations in Academic Laboratories; The Safety Culture in
Industry; Factors That Are Changing the Culture of Safety; Organization of This Book
Chapter 2: Prudent Planning of Experiments
Levels of Formality in Experiment Planning; Individual Responsibilities for Planning
Experiments; Institutional Policies and Emergency Response Planning; Steps for
Planning an Experiment
Chapter 3: Evaluating Hazards and Assessing Risks in the Laboratory
Sources of Information; Toxic Effects of Laboratory Chemicals; Flammable, Reactive,
and Explosive Hazards; Physical Hazards; Biohazards; Hazards from Radioactivity
Chapter 4: Management of Chemicals
Source Reduction; Acquisition of Chemicals; Inventory and Tracking of Chemicals;
Storage of Chemicals in Stockrooms and Laboratories; Recycling of Chemicals,
Containers, and Packaging
Chapter 5: Working With Chemicals
Prudent Planning; General Procedures for Working with Hazardous Chemicals; Working
with Substances of High Toxicity; Working with Biohazardous and Radioactive
Materials; Working with Flammable Chemicals; Working with Highly Reactive or
Explosive Chemicals; Working with Compressed Gases
Chapter 6: Working with Laboratory Equipment
Working with Water-Cooled Equipment; Working with Electrically Powered Laboratory
Equipment; Working with Compressed Gases; Working with High/Low Pressures and
Temperatures; Using Personal Protective, Safety, and Emergency Equipment;
Emergency Procedures
Chapter 7: Disposal of Waste
Chemically Hazardous Waste; Multihazardous Waste; Procedures for the Laboratory-
Scale Treatment of Surplus and Waste Chemicals

CHEMICAL HYGIENE PLAN (rev. 7/03/2012)

Page 17

Chapter 8: Laboratory Facilities
Laboratory Inspection Programs; Laboratory Ventilation; Room Pressure Control
Systems; Special Systems; Maintenance of Ventilation Systems
Chapter 9: Governmental Regulation of Laboratories
Risk and Regulation; The OSHA Laboratory Standard: Occupational Exposure to
Hazardous Chemicals in Laboratories; The Resource Conservation and Recovery Act;
The Clean Air Act; SARA Title III, Community Right-To-Know and Emergency
Notification and Response; The Toxic Substances Control Act; Regulation of Laboratory
Design and Construction

B. Internet Resources
Laboratory Chemical Safety Summaries
http://www.hhmi.org/science/labsafe/lcss/start.htm
Material Safety Data Sheets
http://hazard.com/msds
OSHA Regulations and Technical Information
http://www.osha-slc.gov


CHEMICAL HYGIENE PLAN (rev. 7/03/2012)

Page 18

Appendix A: Laboratory Specific Chemical Hygiene
Procedures
1) Lab Supervisor: __________________ CHO __________________
2) Chemical Users (names or group): __________________ __________________
__________________
3) Department(s): __________________ __________________
4) Laboratory Location: Room _________ Building __________________
5) Material(s) or Hazard Group:
__________________ __________________
a) Quantity used (in the next year):
<1 liter or 100 gm
1 liter/100 gm to 5 liter/1 kg
5 liter/1 kg to 20 liter/5 kg
> 20 liter/5 kg
b) Concentration used: Dilute (<5%) Intermediate (5-25%) Concentrated (>25%)
6) What hazard(s) does these material(s) present?
Flammability Corrosivity
Reactivity Acute Toxicity
Chronic Toxicity Carcinogenicity/Teratogenicity/Mutagenicity
7) Chemical Safety Information and Training
Is safety information for these materials available? Yes No
Has training in the safe use of these materials been provided to all potential users?

Yes No
8) What control measures are necessary to use the material(s) safely?
a) Engineering Controls (indicate room number and hood to be used):
Fume hood
Biosafety cabinet
Gas monitors

CHEMICAL HYGIENE PLAN (rev. 7/03/2012)

Page 19

b) Personal Protective Equipment:
Lab coats
Proper Gloves
Eye protection
Respiratory protection
c) Emergency Response Equipment:
Safety shower Eyewash
Spill control equipment
Fire extinguisher
9) How will user exposure to these chemicals be assessed?
Professional judgment of lab supervisor Air sampling
Other __________________
10) Is medical monitoring required for users of these materials? Yes No
11) Where and with what equipment are the material(s) to be stored?
Room __________________
General storage
Spark-proof refrigerator/freezer
Flammables cabinet
Corrosives cabinet
12) Method of disposal
Chemical waste pick-up Neutralized or consumed during process
Methods used to minimize generation of hazardous waste:



_______________________________________
As part of your protocol planning effort, please identify ways which you can minimize the
amount of chemical waste generated and attach a short narrative description of these
methods to this form.
I accept responsibility for the proper use of these materials in the labs named above
and have assigned chemical hygiene responsibilities within the lab to people with
appropriate training and /or experience.

Lab Supervisor Signature __________________ Date __________________

CHEMICAL HYGIENE PLAN (rev. 7/03/2012)

Page 20

Instructions for Chemical Hygiene Procedures Form
1) Lab Supervisor
Identify the person responsible for the overall conduct of the work involving the chemical
above. Identify the Chemical Hygiene Officer (CHO). If no CHO is identified, the lab
supervisor will serve in that role.
2) Chemical Users
Identify the people or the group who will be actually using the materials. If a group of
short-term workers will perform the work, indicate this and the individual who will
supervise the work.
3) Department(s)
Identify the department(s) under whose control this work will be done.
4) Laboratory Location
Identify the room and building this material will be used in.
5) Material(s) or Hazard Group
Identify the materials to be used by:
1) The chemical names, or
2) If several chemicals from within one of the hazard groups (e.g., corrosives,
flammables, toxics, reactives, radioactives, etc.) a single form can be filled out for
the group. Identify the numbers of the materials to be used within the group,
3) Quantity to be used: Check a range for the approximate quantity of the
chemical(s),
4) Concentration: Check a range for the approximate concentration of the
material(s).
6) What hazard(s) does the material(s) above present?
Identify the hazards that the material(s) named present.
7) Chemical Safety Information
Appropriate safety information for the materials used consists of laboratory
chemical safety summaries, material safety data sheets or other technical
literature that provides the information necessary to anticipate and provide
protection against the hazards associated with the material. Contact the

CHEMICAL HYGIENE PLAN (rev. 7/03/2012)

Page 21

Chemical Storage and Distribution Facility (3555) for help in gathering and
interpreting this information. Training for those who will or might be exposed to
this substance and the specific hazards associated with the material is also
required.
8) What control measures are necessary to use the material(s) safely?
List those pieces of lab equipment necessary for the use of this chemical in the
quantity and concentration indicated. See the Mines Chemical Hygiene Plan for
general criteria for use of these measures.
9) How will user exposure to these chemicals be assessed?
The OSHA Lab Standard requires that worker exposure to all hazardous
chemicals be assessed before work begins and during lab operations if
necessary. Indicate how worker exposure will be assessed. Describe "Other" if
used.
10) Is medical monitoring required for users of the material(s)?
OSHA requires medical monitoring for workers using many different chemicals,
including asbestos, formaldehyde and lead. For complete information about
these requirements, contact the Environmental Health & Safety Department.
11) Where are the(se) materials stored?
Indicate the room and type of storage area where these materials will be stored.
12) Method of disposal
The proper disposal method for all chemical waste is through the chemical waste
pick-up request system. The School is required by law to demonstrate its efforts
to minimize the amount of hazardous waste it generates. List the methods that
will be used to minimize generation of hazardous waste.

CHEMICAL HYGIENE PLAN (rev. 7/03/2012)

Page 22


PERMIT FOR USE OF NON-STANDARD LABORATORY OR
SPECIAL OPERATING PROCEDURES
This permit shall be utilized for all laboratory activities which do not follow standard or
special operating procedures and which thus require pre-approval by the laboratory
supervisor.

Location of Work: Building _____________ Room______

Occurrence (date(s)): _________ to ________

Authorized Personnel: ______________ ________________

Unusual Circumstances:

___ Working alone
___ Working after hours
___ Unattended Operations
___ Other (explain): ____________________________________________________

Hazards Present

____ Highly toxic chemicals
____ Shock sensitive Explosive chemicals
____ Flammable chemicals
___ Other (explain): ____________________________________________________

Special Conditions Required:
____ Spill Containment
____ Designated area (secured from general access; hazards signage)
____ Regular communication with _______________
___ Other (explain):_____________________________________________________






Approved by (one signature required, copy to other person):


_______________________________________
_________
Laboratory Supervisor/Chemical Hygiene Officer
Date

CHEMICAL HYGIENE PLAN (rev. 7/03/2012)

Page 23

Appendix B: Work with High Hazard Chemicals
Table 1: Carcinogens, Reproductive Toxins or Highly Toxic
Chemicals
The chemicals listed below are extremely hazardous due to their toxic effects. This is
not an exclusive list, and may be expanded, based on the professional judgment of the
laboratory supervisor. Workers must have knowledge of the dangers of these chemicals
prior to use, and documentation of training in safe working procedures.
Biologically active compounds
·protease inhibitors (e.g. PMSF, Aprotin, Pepstatin A, Leopeptin);
·protein synthesis inhibitors (e.g. cycloheximide, Puromycin);
·transcriptional inhibitors (e.g. a-amanitin and actinomycin D);
·DNA synthesis inhibitors (e.g. hydroxyurea, nucleotide analogs (i.e. dideoxy
nucleotides), actinomy cin D, acidicolin);
·phosphatase inhibitors (e.g. okadaic acid);
·respiratory chain inhibitors (e.g. sodium azide);
·kinase inhibitors (e.g. NaF);
·mitogenic inhibitors (e.g. colcemid); and
·mitogenic compounds (e.g. concanavalin A).
Castor bean (Ricinus communis) lectin: Ricin A, Ricin B, RCA toxins
Diisopropyl fluorophosphate: highly toxic cholinesterase inhibitor; the antidote, atropine
sulfate and 2-PAM (2-pyridinealdoxime methiodide) must be readily available
N-methyl-N'-nitro-N-nitrosoguanidine: carcinogen (this chemical forms explosive
compounds upon degradation)
Phalloidin from Amanita Phalloides: used for staining actin filaments
Retinoids: potential human teratogens
Streptozotocin: potential human carcinogen
Urethane (ethyl carbamate): an anesthetic agent, potent carcinogen and strong
teratogen, volatile at room temperature

CHEMICAL HYGIENE PLAN (rev. 7/03/2012)

Page 24

Table 2: Shock Sensitive and Pyrophoric Chemicals
The classes of chemicals listed below may explode when subjected to shock or friction.
Therefore containers must have appropriate labels regarding the formation of peroxide
hazards on concentration.
Do not distill or evaporate these materials without first testing for the presence of
peroxides (discard or test for peroxides after 6 months)
·acetaldehyde di·diethylene glycol dimethyl ether (diglyme)
·dioxane
·ethylene glycol dimethyl ether (glyme)
·ethylene glycol ether acetates
·ethylene glycol monoethers (cellosolves)
·furan
·methylacetylene
·methylcyclopentane


CHEMICAL HYGIENE PLAN (rev. 7/03/2012)

Page 25