# CBEN460 & CBEN461 Bioprocess Engineering & LAB

Instructor | John Jechura |

Class Semester Class Location Class Hours |
Fall 2017 AH 330 Mon/Wed/Fri 2:00 pm to 2:50 pm |

Office Location Office Hours |
AH 437 Mon 3:00 - 4:00, Tue & Thu 2:00 - 4:00, Wed 1:00 - 2:00, & by appointment |

Phone | 303-273-3197 |

Mines e-mail address | |

Syllabus (CBEN 460 Lecture) Syllabus |

Page Revisions

December 8. Posted solution for Homework #11.

December 6. Reposted solution for Homework #10 (fixed solution for the 1st problem to use 80 kg/week instead of 85 kg/week).

November 29. Posted solution for Homework #10.

November 27. Posted complete form of slides for Downstream Processing & posted solution to Quiz #6.

November 17. Posted Homework #11.

November 16. Posted solution to Homework #9. Posted Homework #10.

November 15. Posted draft notes for Unit Operations (drying & filtration).

November 13. Posted a corrected solution for Quiz #5 (the slope for half the rate was drawn incorrectly). Posted notes for fluid flow & heat transfer.

November 11. Posted solution for Homework #8 & Quiz #5.

November 3. Posted Homework #8 & #9. Posted correction to Homework #7 (dimensions for problem #2 were incorrect).

November 2. Posted solution for Homework #7.

October 25. Posted comments for Chapter 10.

October 18. Posted Homework #7.

October 17. Posted notes for productivity in batch & continuous reactors.

October 14. Solution for Homework #6 posted.

October 11. Solution for Homework #5 posted.

October 10. Created section for laboratory tasks.

October 9. Homework #6 revised - made additional option for 2nd problem. Posted Quiz #4. Posted comments on cell growth models.

October 6. Homework #6 revised - gave an optional problem.

October 4. Posted solution for Homework #4 & Quiz #3.

September 25. Posted notes for enzymes.

September 22. Posted solutions for Homework #3 & Quizzes #1 & #2. Posted statements for Homeworks #5 & #6.

September 20. Posted comments for Energy Balances.

September 18. Posted Homework #4 & supplemental information under the Energy Balance section.

September 15. Posted solution for Homework #2.

September 6. Posted revised instructions for Homework #2.

September 2. Posted Homework #2.

September 1. Posted solution to Homework #1.

August 28. Posted notes for solving simultaneous linear equations & meaning of half reactions.

August 23. Posted Homework #1 & due date.

August 2017. Content initialized for Fall Semester.

Lecture topic outline (associated chapters in text; supplemental text will be made available to class).

Introduction & Basics

- Material Properties & Stoichiometry (Chapter 7)
- Solving simultaneous linear equations: use of Cramer's rule & using capabilities in Excel (sample spreadsheet)
- Half reactions
- Transient Material Balances (Chapter 9)
- Energy Balances (Supplemental Chapter 5)
- Data Analysis (Supplemental Chapter 3)
- Enzyme Kinetics (Chapter 3)
- Cell Growth & Decay Kinetics (Chapter 6)

- Productivity in Batch & Continuous Bioreactors (Chapter 9)
- Considerations for going between lab & industrial scale of bioreactors (Chapter 10)
- Tank Mixing, Agitation, & Aeration
- Scale-up
- Sterilization
- Physical Processes
- Fluid & Slurry Flow (Supplemental Chapter 7)
- Heat Transfer (Supplemental Chapter 9)
- Mass Transfer & Transport Phenomena (Supplemental Chapter 10)
- Recovery & Purification of Products (Chapter 11 & Supplemental Chapter 11)
- Moisture Content, Drying, & Evaporation
- Filtration & Centrifugation
- Flocculation & Coagulation
- Purification & Recovery Technologies
- Instrumentation & Control (Chapter 10)

Laboratory topics

Group assignments

Computer Labs

- Hydrometer Analysis of a Sugar/Ethanol/Water Solution
- Optimization of Temperature in Enzymatic Reactors (Batch & Chemostat)

- Extraction of Essential Oils
- Biodiesel production
- Fermentation

Extra instructor notes

Useful VBA (Visual Basic for Applications) code (notes for downloading):

- Linear interpolation
- Spline interpolation
- Interpolation of distillation curves internally using the probability function transformation of yield values. Code allows this to be done using linear interpolation or spline interpolation (on a temperature vs. transformed yield basis).

- The code was originally developed to run with Excel 2000. It has been tested & it runs properly with Excel 2007, 2010, & 2013. Here are notes to incorporate the VBA macros into an Excel 2010 spreadsheet. Below are other sites that describe what needs to be done to allow the macros to work.
- http://support.microsoft.com/kb/919195
- http://www.dummies.com/how-to/content/how-to-set-the-macro-security-level-in-excel-2007.html#comments
- There have been issues if one wants to use these macros on a Mac. Excel 2004 has VBA support built-in but Excel 2008 did not. The latest version are supposed to have VBA support.
- If you are using a version of Excel (or some other spreadsheet) that cannot run these macros then here is a sample spreadsheet for doing the interpolation calculations without requiring macros (the user will have to be careful to copy cells with formulas as necessary). There are separate sheets for linear interpolation (arbitrary X & Y values) and interpolation of cumulative yield data (°F vs. yield%).

Homework Assignments

Homework assignments are to be emailed to the instructor usually in spreadsheet (Excel) format. The following rules must be followed:

- There is to be a summary page with all answers in the order & units requested. Values in the summary are not to be copied by values but rather linked to any additional pages where the calculations are actually performed.
- All spreadsheets are to be self contained, i.e., there are to be no links to other spreadsheets.

A sample spreadsheet with the preferred format is here.

Due Date & Link to Problem Statement | Topic & Link to Solution | |

#1 | Thursday Aug 31 | Stoichiometery |

#2 | Tuesday, Sept 12 Thu Sept 14 | Material balances around bioreactors |

#3 | Thu Sept 21 | Data analysis & regression |

#4 | Tue Oct 3 | Energy Balances |

#5 | Tue Oct 10 | Enzymes |

#6 | Fri Oct 13 | Cell Growth |

#7 | Tue Oct 31 Wed Nov 1 | Other bioreactor consideratios: scale up, sterilization, ... |

#8 | Fri Nov 10 | Fluid flow |

#9 | Wed Nov 15 | Heat transfer |

#10 | Tue Nov 28 | Downstream processing, filtration, & drying |

#11 | Thu Dec 7 | Downstream processing |

Quizzes

Quiz Date | Topic & Link to Solution | |

#1 | Sept 6 | Stoichiometry |

#2 | Sept 20 | Chemostat performance |

#3 | Oct 2 | Model parameters from data |

#4 | Oct 6 | Model parameters from data |

#5 | Nov 10 | Productivity from chemostat |

#6 | Nov 20 | Heat transfer |

#7 | TBD | |

#8 | TBD |

Exams

Exam Date | Link to Solution | |

#1 | Wed Oct 25 | Introduction & Basics |

#2 | TBD | |

Final Exam | TBD |

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